Program Version: 1.04 (5 May 2015) Program Started: 05/21/2015 19:13:08 Microcosm ports: Algae: 1 Digestor: 2 Sample loop purge time for CH4mix (min): 12.00 Sample loop purge time for Air (min): 6.00 Sample loop purge time for Algae (min): 6.00 Sample loop purge time for N2 (min): 6.00 Sample loop purge time for Dig (min): 6.00 Loop time (hr): 5.00 --------------------------- Experiment Description ---------------------------- Have just added sterilized medium to reactors, but have not inoculated Reactors are starting out both aerobic, and dilution rate is 1/d Still much to do ------------------------------------------------------------------------------- Program Version: 1.04 (5 May 2015) Program Started: 05/22/2015 13:31:56 Microcosm ports: Algae: 1 Digestor: 2 Sample loop purge time for CH4mix (min): 12.00 Sample loop purge time for Air (min): 6.00 Sample loop purge time for Algae (min): 6.00 Sample loop purge time for N2 (min): 6.00 Sample loop purge time for Dig (min): 6.00 Loop time (hr): 5.00 --------------------------- Experiment Description ---------------------------- Restarting program after renaming files to Exp1 from Exp1_pre This was done because algal reactor was inoculated with 1 L of Chlamydomonas reinhartii CW15 at approximately 12:30 today Currently, exchange flow between reactors is zero Gas flow of Air and N2 are both set to 20 mL/min Agitation at 10 rpm Need to calibrate analyzers and fill rest of medium to digestor ------------------------------------------------------------------------------- Note Number: 1 (05/22/2015 13:35:55) Turned off exchange after inoculating. Allow algal culture to grow first ------------------------------------------------------------------------------- Note Number: 2 (05/22/2015 13:36:43) ------------------------------------------------------------------------------- Note Number: 3 (05/22/2015 13:37:33) Have not measured light level yet. Let level is 1 bank of metal halide and 1 for high pressure sodium. One of each bulb is on. ------------------------------------------------------------------------------- Note Number: 4 (05/22/2015 14:51:29) recalibrated analzyers, and measured composition of airtank 1 ------------------------------------------------------------------------------- Note Number: 5 (05/22/2015 14:52:33) Added the final amount of medium to digestor ------------------------------------------------------------------------------- Note Number: 6 (05/22/2015 14:54:28) All data have been updated, so sample reactors. Note, because of filling digestor with the last 2 L caused a lot of N2 from the digestor to be carried over to the algal tank, so this measurements will likely show low O2 in head space ------------------------------------------------------------------------------- Program Version: 1.04 (5 May 2015) Program Started: 05/22/2015 17:12:30 Microcosm ports: Algae: 1 Digestor: 2 Sample loop purge time for CH4mix (min): 12.00 Sample loop purge time for Air (min): 6.00 Sample loop purge time for Algae (min): 6.00 Sample loop purge time for N2 (min): 6.00 Sample loop purge time for Dig (min): 6.00 Loop time (hr): 5.00 --------------------------- Experiment Description ---------------------------- Added ability to run shell and export images ------------------------------------------------------------------------------- Program Version: 1.04 (5 May 2015) Program Started: 05/24/2015 21:31:50 Microcosm ports: Algae: 1 Digestor: 2 Sample loop purge time for CH4mix (min): 12.00 Sample loop purge time for Air (min): 6.00 Sample loop purge time for Algae (min): 6.00 Sample loop purge time for N2 (min): 6.00 Sample loop purge time for Dig (min): 6.00 Loop time (hr): 5.00 --------------------------- Experiment Description ---------------------------- restart after crash changing gas flow rate ------------------------------------------------------------------------------- Note Number: 7 (05/24/2015 21:33:59) Increased flow rate in algae tank to 50 mL/min. Resampling ------------------------------------------------------------------------------- Note Number: 8 (05/25/2015 09:01:18) Increase flow rate to algal culture to 100 mL/min because it has gone anaerobic. Phototrophs are not doing their job, or perhaps the lights are not coming on. ------------------------------------------------------------------------------- Program Version: 1.04 (5 May 2015) Program Started: 05/26/2015 10:20:38 Microcosm ports: Algae: 1 Digestor: 2 Sample loop purge time for CH4mix (min): 12.00 Sample loop purge time for Air (min): 6.00 Sample loop purge time for Algae (min): 6.00 Sample loop purge time for N2 (min): 6.00 Sample loop purge time for Dig (min): 6.00 Loop time (hr): 5.00 --------------------------- Experiment Description ---------------------------- Restarting from 647c string read crash...need to fix this ------------------------------------------------------------------------------- Note Number: 7 (05/26/2015 10:22:57) deacreased air flow back down to 20 mL/min ------------------------------------------------------------------------------- Program Version: 1.04 (5 May 2015) Program Started: 05/26/2015 11:12:20 Microcosm ports: Algae: 1 Digestor: 2 Sample loop purge time for CH4mix (min): 12.00 Sample loop purge time for Air (min): 6.00 Sample loop purge time for Algae (min): 6.00 Sample loop purge time for N2 (min): 6.00 Sample loop purge time for Dig (min): 6.00 Loop time (hr): 5.00 --------------------------- Experiment Description ---------------------------- Restarting after minor program changes to fix 647c reads ------------------------------------------------------------------------------- Program Version: 1.06 (26 May 2015) Program (Re)Started: 05/26/2015 11:52:48, time = 5.49500 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 5.00 --------------------------- Experiment Description ---------------------------- Restart after a few minor fixes ------------------------------------------------------------------------------- Note Number: 8 (05/26/2015 11:54:30 time = 5.49618 d) Testing note routine ------------------------------------------------------------------------------- Note Number: 9 (05/26/2015 11:55:25 time = 5.49682 d) No change, just testing communication ------------------------------------------------------------------------------- Program Version: 1.06 (26 May 2015) Program (Re)Started: 05/26/2015 22:38:54, time = 5.94368 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting after decreasing sample time to 2 hrs from 5 ------------------------------------------------------------------------------- Note Number: 10 (05/27/2015 14:16:10 time = 6.59456 d) Growth chamber time was ~ 4 hours slow. It was updated to the correct EDT at 14:10 27 May 2014 ------------------------------------------------------------------------------- Note Number: 11 (05/28/2015 12:41:03 time = 7.52851 d) RPM of algal reactor increased from 10 to 60 ------------------------------------------------------------------------------- Note Number: 12 (05/28/2015 13:19:17 time = 7.55506 d) Note, increased RPM right before sample was taken. ------------------------------------------------------------------------------- Note Number: 13 (05/28/2015 18:10:12 time = 7.75708 d) Decrease air flow to algal reactor to 5 mL/min ------------------------------------------------------------------------------- Note Number: 14 (06/10/2015 16:09:18 time = 20.67312 d) Dropped Digestor to 5 mL/min ------------------------------------------------------------------------------- Note Number: 15 (06/30/2015 10:54:49 time = 40.45473 d) 12 ------------------------------------------------------------------------------- Note Number: 16 (06/30/2015 11:07:13 time = 40.46334 d) added sparger (2 micron) and increased flow rate in algal reactor to 20 ml/min ------------------------------------------------------------------------------- Note Number: 17 (07/08/2015 17:40:52 time = 48.73671 d) Decreased flow from 20 to 5 sccm in the algal reactor. ------------------------------------------------------------------------------- Note Number: 18 (07/10/2015 17:13:25 time = 50.71765 d) Increased lights: 2 halides and 2 HP sodium ------------------------------------------------------------------------------- Note Number: 19 (07/14/2015 14:04:58 time = 54.58678 d) Increased flow to Algal reactor to 20 mL/min ------------------------------------------------------------------------------- Note Number: 20 (07/16/2015 13:48:15 time = 56.57517 d) Added CO2 to air feed. Flow rate set to 0.4 mL/min. At 11:40. ------------------------------------------------------------------------------- Program Version: 1.07 (16 Jul 2015) Program (Re)Started: 07/16/2015 17:46:23, time = 56.74054 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Updated program to read 647c parameters more often ------------------------------------------------------------------------------- Program Version: 1.07 (16 Jul 2015) Program (Re)Started: 07/17/2015 11:06:42, time = 57.46299 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restart from crash ------------------------------------------------------------------------------- Note Number: 21 (07/17/2015 12:06:45 time = 57.50469 d) Sampling now to stay on ~ 2 hr period ------------------------------------------------------------------------------- Program Version: 1.07 (16 Jul 2015) Program (Re)Started: 07/17/2015 12:19:49, time = 57.51376 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Fixing 647c com problem... ------------------------------------------------------------------------------- Note Number: 22 (07/19/2015 09:22:38 time = 59.39072 d) Turned CO2 feed off ------------------------------------------------------------------------------- Note Number: 23 (07/20/2015 15:01:21 time = 60.62594 d) Approximately 10 mL of a 4 M K2HPO4 solution was added to algal reactor, bring PO4 concentration up to ~10 mM. pH rapidly increased to 7. ------------------------------------------------------------------------------- Note Number: 24 (07/20/2015 15:46:01 time = 60.65696 d) CO2 (2%) turned back on at 15:07. pH holding well at 7.45 ------------------------------------------------------------------------------- Note Number: 25 (07/22/2015 17:49:43 time = 62.74286 d) Turned reactor exchange flow on for first time. Effective diluution rate for algal reactor is 0.1 1/d ------------------------------------------------------------------------------- Note Number: 26 (07/22/2015 17:50:32 time = 62.74343 d) Added 180 mmoles of potassium phosphate buffer adjusted to pH 7.5. Buffer was autoclaved before adding ------------------------------------------------------------------------------- Note Number: 27 (07/23/2015 09:16:44 time = 63.38662 d) Increased N2 flow to digester to 20 mL/min ------------------------------------------------------------------------------- Note Number: 28 (07/23/2015 09:19:03 time = 63.38823 d) Increaed exchange flow to 4032 mL/min or 1.0 1/d dilution rate ------------------------------------------------------------------------------- Note Number: 29 (07/30/2015 19:11:35 time = 70.79971 d) Added ~500 mL of incoculum to digestor. Groundwater sample from Little Pond near the freshwater input (cypress street) at a depth of 6 m. DO 0 pH ~5.9 Conductivity low ------------------------------------------------------------------------------- Program Version: 1.07 (16 Jul 2015) Program (Re)Started: 08/01/2015 19:38:22, time = 72.81831 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting after computer crash ------------------------------------------------------------------------------- Note Number: 28 (08/01/2015 21:37:47 time = 72.90124 d) Mix tank composition may have changed or CH4 analyzer out of calibration. Setting CH4 to 5.17%, but it should be 4.7 or so ------------------------------------------------------------------------------- Note Number: 29 (08/01/2015 23:05:44 time = 72.96232 d) Going back to assuming mix tank is correct ------------------------------------------------------------------------------- Note Number: 30 (08/04/2015 11:31:14 time = 75.48003 d) Analyzers calibrated. Note, had some problems with calibrating the correct range on the CH4 analyzers, but redid on range 2 (R2). Still says channel 1 is not calibrated, but this is because I may have messed up calibration of range 1, etc, but that's not used Also, CH4 mix tank gives a reading of 4.76%, but this is close enough to what I have been using of 7.74% ------------------------------------------------------------------------------- Note Number: 31 (08/10/2015 12:50:51 time = 81.53531 d) Flow between reactors turned off to measure respiration rate in digestor ------------------------------------------------------------------------------- Note Number: 32 (08/14/2015 11:37:59 time = 85.48471 d) Added 10 mL of the 4 M K2HPO4 buffer to algal reactor only ------------------------------------------------------------------------------- Note Number: 33 (08/21/2015 15:31:51 time = 92.64712 d) Added 0.4 mL of sediments for station P22 core collected from the Parker River in Plum Island estuary. ------------------------------------------------------------------------------- Note Number: 34 (08/28/2015 15:39:54 time = 99.65271 d) Transferred approximately 0.5 L from digestor to algal reactor. This is to makeup volume for samples that have been removed from system for analysis ------------------------------------------------------------------------------- Note Number: 35 (09/01/2015 14:48:32 time = 103.61703 d) Increased N2 flow to digestor to 30 mL/min ------------------------------------------------------------------------------- Note Number: 36 (09/03/2015 13:15:25 time = 105.55238 d) ------------------------------------------------------------------------------- Note Number: 37 (09/03/2015 13:16:47 time = 105.55332 d) Digester inoculated with sediment from Falmouth Sequence Batch wastewater plant ------------------------------------------------------------------------------- Note Number: 38 (09/09/2015 16:04:46 time = 111.66998 d) Added 2.45 g of NaAcetate (trihydrate) added to digestor (1 mM) at 16:04 ------------------------------------------------------------------------------- Note Number: 39 (09/14/2015 17:03:46 time = 116.71095 d) nothing changed, just checking MFC system ------------------------------------------------------------------------------- Note Number: 40 (09/15/2015 09:34:01 time = 117.39862 d) Added 1 mL glucose (3.24 g) to digestor ------------------------------------------------------------------------------- Program Version: 1.07 (16 Jul 2015) Program (Re)Started: 09/15/2015 18:15:38, time = 117.76086 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Crash when attempting to monitor MKS 647c flows. Crash occured first in reading flow ranges, but actual crash occured in monitorMFC routine when attempting to read(iostring), line 2313 in version 1.07 ------------------------------------------------------------------------------- Program Version: 1.07 (16 Jul 2015) Program (Re)Started: 09/15/2015 19:43:00, time = 117.82153 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restart after some com port testing ------------------------------------------------------------------------------- Program Version: 1.07 (16 Jul 2015) Program (Re)Started: 09/16/2015 11:46:47, time = 118.49082 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- testing Com to 647c still ------------------------------------------------------------------------------- Program Version: 1.07 (16 Jul 2015) Program (Re)Started: 09/16/2015 13:51:58, time = 118.57775 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- still working on COM to 647c ------------------------------------------------------------------------------- Program Version: 1.07 (16 Jul 2015) Program (Re)Started: 09/16/2015 15:56:09, time = 118.66399 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- com port work ------------------------------------------------------------------------------- Program Version: 1.07 (16 Jul 2015) Program (Re)Started: 09/16/2015 18:20:47, time = 118.76443 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- continue with com port experiments ------------------------------------------------------------------------------- Program Version: 1.07 (16 Jul 2015) Program (Re)Started: 09/17/2015 13:11:13, time = 119.54946 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting after I messed up testing come on reset MKS 647c to factory defaults!!!! I'm not sure what methane mix was set to, but currently running 100 N2 (MFC#8) and 4.92 CH4 (MFC#7). But, running N2 at 110 sccm ------------------------------------------------------------------------------- Note Number: 34 (09/17/2015 13:25:49 time = 119.55959 d) Just going to sample mode ------------------------------------------------------------------------------- Note Number: 35 (09/18/2015 11:49:49 time = 120.49293 d) After MFC 647c reset, I set the CH4 mixing to 100 sccm N2 with 4.90 sccm CH4 slaved to N2. However, N2 is run at 110 sccm The new mix tank concentration appears to be 4.92% ------------------------------------------------------------------------------- Note Number: 36 (09/18/2015 11:51:39 time = 120.49420 d) Sampling after setting CH4 mix tank concentration ------------------------------------------------------------------------------- Program Version: 1.08 (18 Sep 2015) Program (Re)Started: 09/18/2015 13:21:36, time = 120.55667 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- New version that has improved (?) com to MKS 647c ------------------------------------------------------------------------------- Note Number: 37 (09/18/2015 13:24:56 time = 120.55898 d) just testing com to 647c ------------------------------------------------------------------------------- Note Number: 38 (09/18/2015 13:27:09 time = 120.56052 d) Testing of com to 647c looks good. Return to sampling ------------------------------------------------------------------------------- Program Version: 1.10 (22 Sep 2015) Program (Re)Started: 09/22/2015 17:39:20, time = 124.73565 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- New version of program 1.10 along with modified hardware that will allow the Air+CO2 gas stream being fed to algal reactor to be directly measured ------------------------------------------------------------------------------- Note Number: 39 (09/22/2015 18:55:33 time = 124.78857 d) First measure of CO2+Air mix tank. There is a slight leak in the mix tank but this will get fixed tomorrow ------------------------------------------------------------------------------- Note Number: 40 (09/22/2015 18:56:23 time = 124.78915 d) Sampling after CO2 mix tank measurement ------------------------------------------------------------------------------- Note Number: 41 (09/23/2015 10:11:27 time = 125.42462 d) Turned exchange flow back on to a dilution rate of 1/d or 4L/d ------------------------------------------------------------------------------- Note Number: 42 (09/23/2015 16:20:04 time = 125.68060 d) Increased air+co2 flow to algal reactor from 20 to 30 mL/min ------------------------------------------------------------------------------- Note Number: 43 (09/25/2015 21:55:48 time = 127.91375 d) Increasing Air + CO2 feed to releave near 0 DO levels ------------------------------------------------------------------------------- Note Number: 44 (09/26/2015 14:33:08 time = 128.60634 d) Algal & \\ *Algal reactor and microbial digester decoupled, i.e. pump paused at 14:33 9/26/ ------------------------------------------------------------------------------- Note Number: 45 (09/26/2015 14:56:53 time = 128.62283 d) Flow rate was turned off at 14:33, not 14:57 ------------------------------------------------------------------------------- Note Number: 46 (09/30/2015 09:46:30 time = 132.40729 d) Decreased gas flow to algal reactor from 100 to 30 mL/min ------------------------------------------------------------------------------- Note Number: 47 (09/30/2015 12:19:39 time = 132.51364 d) Returning to 100 mL/min because of high respiration rate in algal reactor ------------------------------------------------------------------------------- Program Version: 1.10 (22 Sep 2015) Program (Re)Started: 10/04/2015 18:09:45, time = 136.75677 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting after changing format of Exp1.oc file ------------------------------------------------------------------------------- Program Version: 1.10 (22 Sep 2015) Program (Re)Started: 10/06/2015 09:04:56, time = 138.37843 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- restarting after addition of WTDAC and WTAIN boards on COM 9 ------------------------------------------------------------------------------- Note Number: 48 (10/06/2015 09:30:01 time = 138.39584 d) Decreased gas flow to algae form 100 to 30 mL/min since net heterotrophy appears much deacreased ------------------------------------------------------------------------------- Program Version: 1.12 (6 Oct 2015) Program (Re)Started: 10/06/2015 17:20:16, time = 138.72241 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting with version 1.12, which adds ability for computer control of cole-parmer exchange pump and also adds two point calibration of CO2 measurement. This required a new format for the Exp1.dat file, as well as the tecplot files, and the CSV file ------------------------------------------------------------------------------- Note Number: 49 (10/06/2015 17:27:19 time = 138.72731 d) Testing control of exchange pump, which appears to be working ------------------------------------------------------------------------------- Note Number: 50 (10/06/2015 17:30:48 time = 138.72972 d) Pump control working, so sample system ------------------------------------------------------------------------------- Program Version: 1.12 (6 Oct 2015) Program (Re)Started: 10/13/2015 18:12:28, time = 145.75866 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting after fixing errors in the Exp1.dat file. Mostly assoicated with errors in reading gas flow rates in old version of MonitorA2M and adding in estimated CO2 addition ------------------------------------------------------------------------------- Program Version: 1.12 (6 Oct 2015) Program (Re)Started: 10/13/2015 21:15:43, time = 145.88591 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting after error in Exp1.dat Tecplot header record. ------------------------------------------------------------------------------- Note Number: 51 (10/18/2015 10:20:06 time = 150.43062 d) Calculations indicate that glucose + Ac have been consumed and the CH4 is trending down, so time to couple the reactors ------------------------------------------------------------------------------- Note Number: 52 (10/23/2015 09:38:41 time = 155.40187 d) Reduced flow rate to 0.05 1/d (0.2 L/d) to alleviate low DO in algal reactor ------------------------------------------------------------------------------- Note Number: 53 (10/23/2015 09:39:52 time = 155.40268 d) Return to sampling after reducing exchange flow rate ------------------------------------------------------------------------------- Note Number: 54 (10/27/2015 21:31:36 time = 159.89694 d) Reduced flow rate to 0.01 1/d or 0.04 L/d ------------------------------------------------------------------------------- Note Number: 55 (10/29/2015 10:24:44 time = 161.43384 d) Increase gas flow to algal reactor to 100 mL/min ------------------------------------------------------------------------------- Note Number: 56 (11/07/2015 21:38:27 time = 170.90170 d) Setting flow to zero ------------------------------------------------------------------------------- Note Number: 57 (11/10/2015 10:32:10 time = 173.43901 d) Reduced air flow because air tank is running low. ------------------------------------------------------------------------------- Note Number: 58 (11/10/2015 11:37:00 time = 173.48402 d) Added 10 mL of 4 M K2HPO4 to algal reactor. This increased pH to 6.97. Also, changed direction of impeller but Not RPM ------------------------------------------------------------------------------- Note Number: 59 (11/10/2015 21:35:32 time = 173.89967 d) Returned air + CO2 flow rate to 100 mL/min due to low DO ------------------------------------------------------------------------------- Note Number: 60 (11/10/2015 21:37:07 time = 173.90077 d) Changed gas flow rate, so OK so sample immediately ------------------------------------------------------------------------------- Note Number: 61 (11/12/2015 11:08:37 time = 175.46431 d) Decrased gas flow to algal reactor to 30 mL/min because tank is running low ------------------------------------------------------------------------------- Program Version: 1.12 (6 Oct 2015) Program (Re)Started: 11/12/2015 11:13:13, time = 175.46751 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Changed maxflow exchange from 4L/d to 1 L/d on pump and recovery file ------------------------------------------------------------------------------- Note Number: 62 (11/12/2015 11:16:03 time = 175.46948 d) testing new max flow exchange rate. Reactors still uncoupled ------------------------------------------------------------------------------- Note Number: 63 (11/12/2015 11:16:41 time = 175.46992 d) just getting sampling going ------------------------------------------------------------------------------- Note Number: 64 (11/13/2015 10:51:26 time = 176.45238 d) New air tank on line, so back to 100 mL/min flow. However, new tank only half full at ~1200 psig. ------------------------------------------------------------------------------- Note Number: 65 (11/17/2015 17:51:10 time = 180.74387 d) Place air tank #3 online, calibrated gas analyzers, and measured composition of air tank #3. Pressure starts at 2660 psig ------------------------------------------------------------------------------- Note Number: 66 (11/17/2015 17:52:12 time = 180.74458 d) Sampling after changing air tank ------------------------------------------------------------------------------- Note Number: 67 (12/04/2015 16:00:50 time = 197.66725 d) Decreasing gas flow to algal reactor to 30 mL/min from 100 mL/min ------------------------------------------------------------------------------- Note Number: 68 (12/11/2015 10:58:03 time = 204.45699 d) Added 4.5 g FeCl3 to digestor to test for Fe stimulation of methanogenesis ------------------------------------------------------------------------------- Note Number: 69 (12/15/2015 16:51:08 time = 208.70218 d) reduced flow to digestor from 30 to 5 mL/min ------------------------------------------------------------------------------- Program Version: 1.20 (23-Dec-2015) Program (Re)Started: 12/23/2015 15:26:50, time = 216.64363 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting with version 1.20. This version sets a minimum flow into the bioreactors during sampling. ------------------------------------------------------------------------------- Note Number: 70 (01/15/2016 17:22:03 time = 239.72365 d) Air tank #4 online at 16:30 15 Jan 2016; 2550 psig Analyzers calibrated ------------------------------------------------------------------------------- Note Number: 71 (01/19/2016 10:43:41 time = 243.44701 d) Turned exchange flow between reactors on at a dilution rate of 0.05 1/d or 0.2 L/d ------------------------------------------------------------------------------- Note Number: 72 (01/29/2016 12:17:27 time = 253.51212 d) Turned of flow between reactors to install UV sterilizers ------------------------------------------------------------------------------- Note Number: 73 (02/02/2016 17:03:58 time = 257.71109 d) New N2 tank on-line, 2300 psig ------------------------------------------------------------------------------- Note Number: 74 (02/17/2016 19:34:12 time = 272.81543 d) New N2 tank online 17 Feb 2016, 19:30 (2300 psig) ------------------------------------------------------------------------------- Program Version: 1.20 (23-Dec-2015) Program (Re)Started: 02/22/2016 18:37:09, time = 277.77580 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting after installing UV in-line sterilizers Also, computer monitoring all got shut down strangely somehow ------------------------------------------------------------------------------- Note Number: 72 (02/22/2016 18:40:48 time = 277.77832 d) Flow rate turned on a 0.2 L/d, or a dilution rate of 0.05 1/d based on algal bioreactor volume ------------------------------------------------------------------------------- Note Number: 73 (03/03/2016 10:23:07 time = 287.43271 d) Increased flow to 0.4 L/d or 0.1 1/d ------------------------------------------------------------------------------- Note Number: 74 (03/04/2016 11:34:30 time = 288.48230 d) New N2 tank on line (2200 psig) ------------------------------------------------------------------------------- Note Number: 75 (03/29/2016 19:27:32 time = 313.81079 d) Changed back to previous N2 tank that has 1000 psig ------------------------------------------------------------------------------- Note Number: 76 (04/05/2016 10:33:21 time = 320.43982 d) Changed to air tank #5. 2500 psig. Also changed N2 Tank, 2250 psig ------------------------------------------------------------------------------- Note Number: 77 (04/05/2016 10:34:15 time = 320.44046 d) Left sampling to enter new air tank #5 composition. So OK to sample now ------------------------------------------------------------------------------- Note Number: 78 (04/05/2016 11:45:00 time = 320.48959 d) Increased flow to 8.2 L/d or 0.2 1/d dilution rate ------------------------------------------------------------------------------- Note Number: 79 (04/21/2016 10:33:05 time = 336.43964 d) New N2 Tank on-line, 2300 psig ------------------------------------------------------------------------------- Note Number: 80 (04/29/2016 11:52:25 time = 344.49472 d) turned flow off to install cell distrupter pump, but also found out that the UV flow tubes had become plugged, so NO flow had been exchanging between the reactors. The UV sources were taken out of loop for now. ------------------------------------------------------------------------------- Note Number: 81 (04/29/2016 11:55:29 time = 344.49686 d) problem with reading pump. Going to restart system ------------------------------------------------------------------------------- Program Version: 1.20 (23-Dec-2015) Program (Re)Started: 04/29/2016 11:57:20, time = 344.49815 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting after problem with reading pump port ------------------------------------------------------------------------------- Note Number: 82 (04/29/2016 12:01:10 time = 344.50082 d) ------------------------------------------------------------------------------- Note Number: 83 (04/29/2016 12:03:39 time = 344.50253 d) ------------------------------------------------------------------------------- Program Version: 1.20 (23-Dec-2015) Program (Re)Started: 04/29/2016 12:56:19, time = 344.53911 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting after problems with Weeder Tech comm ------------------------------------------------------------------------------- Note Number: 84 (04/29/2016 13:00:53 time = 344.54227 d) Returned to normal operation of 0.8 L/d. Problem before may have been that the pause button had been pressed, so flow rate was actually zero as being reported. ------------------------------------------------------------------------------- Note Number: 85 (04/29/2016 13:11:37 time = 344.54974 d) No need to wait for sampling ------------------------------------------------------------------------------- Note Number: 86 (05/03/2016 10:31:13 time = 348.43835 d) Increased flow to 1 L/d or 0.25 1/d ------------------------------------------------------------------------------- Note Number: 87 (05/06/2016 14:56:29 time = 351.62256 d) Replaced N2 tank at 14:55, 6 May 2016, 2300 psig ------------------------------------------------------------------------------- Program Version: 1.20 (23-Dec-2015) Program (Re)Started: 05/06/2016 14:58:57, time = 351.62427 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting after increasing max exchange flow rate from 1 L/d to 4 L/d in Exp1.rcv file ------------------------------------------------------------------------------- Note Number: 88 (05/06/2016 15:03:26 time = 351.62738 d) Increased max exchange flow, but there may be bug in program, because when I now set it to 1.0 it comes back as 4, but the pump reads 1. ------------------------------------------------------------------------------- Note Number: 89 (05/06/2016 15:04:44 time = 351.62830 d) still problem with reading flow correctly. ------------------------------------------------------------------------------- Note Number: 90 (05/06/2016 15:21:15 time = 351.63977 d) Changed flow rate to 2.0 L/d, or D = 0.5 1/d. Note, problem in reading pump value was not in program, but rather I forgot to also set the pump output voltage to 10V = 4 L/d. ------------------------------------------------------------------------------- Note Number: 91 (05/06/2016 15:22:30 time = 351.64062 d) OK to measure now. Increased exchange flow to 2 L/d ------------------------------------------------------------------------------- Note Number: 92 (05/09/2016 10:49:31 time = 354.45105 d) Increased flow to 4 L/d or D = 1.0 1/d ------------------------------------------------------------------------------- Note Number: 93 (05/12/2016 10:11:02 time = 357.42432 d) Added 7 mL of 4 M K2HPO4 buffer to algal reactor ------------------------------------------------------------------------------- Note Number: 94 (05/18/2016 18:24:49 time = 363.76724 d) Added 30 mL of 4 M K2HPO4 ------------------------------------------------------------------------------- Note Number: 95 (05/19/2016 10:53:17 time = 364.45367 d) Increase RPM in digestor to 30 RPM from 10. ------------------------------------------------------------------------------- Note Number: 96 (05/20/2016 14:27:01 time = 365.60208 d) New N2 tank online, 2330 psig. Note, old tank still as 350 psig, so keeping for now. ------------------------------------------------------------------------------- Note Number: 97 (05/31/2016 09:38:11 time = 376.40152 d) Added 90 mL of K2HPO4 ------------------------------------------------------------------------------- Note Number: 98 (06/03/2016 09:54:43 time = 379.41299 d) N2 tank went empty at some point, so digester sparging and CH4 calibration had issue. Replaced N2 tank at 9:55 3-Jun-2016 ------------------------------------------------------------------------------- Note Number: 99 (06/09/2016 10:54:45 time = 385.45468 d) New N2 tank on-line 2275 psig ------------------------------------------------------------------------------- Note Number: 100 (06/20/2016 11:21:36 time = 396.47333 d) No change to flow rate, as currently at max. ------------------------------------------------------------------------------- Note Number: 101 (06/27/2016 09:00:00 time = 403.37500 d) Noticed the N2 tank empty (forgot to change on Fri) at 9:00 on 27 June 2016. Holding here until N2 tank can be replaced. New N2 Tank on line at 10:20 27-Jun-2016. Need to purge CH4 mix tank for a bit first ------------------------------------------------------------------------------- Note Number: 102 (06/27/2016 11:24:44 time = 403.47549 d) CH4 mix tank as been purged for 45 min, so sampling can begin again ------------------------------------------------------------------------------- Note Number: 103 (06/28/2016 11:15:05 time = 404.46881 d) New air tank #6 online at 10:40 28Jun2016, 2550 psig ------------------------------------------------------------------------------- Program Version: 1.20 (23-Dec-2015) Program (Re)Started: 07/15/2016 14:55:20, time = 421.62176 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting after increasing max exchange pump flow to 8 L/d ------------------------------------------------------------------------------- Note Number: 104 (07/15/2016 14:56:51 time = 421.62280 d) Increased exchange flow to 6.0 L/d or dilution rate of 1.5 1/d ------------------------------------------------------------------------------- Note Number: 105 (07/15/2016 14:59:21 time = 421.62454 d) Increased exchange flow to 6 L/d or 1.5 1/d dilution rate ------------------------------------------------------------------------------- Note Number: 106 (07/28/2016 09:51:15 time = 434.41058 d) Increased flow to 2.0 1/d or 8 L/d ------------------------------------------------------------------------------- Note Number: 107 (07/30/2016 09:09:51 time = 436.38184 d) Reduced flow back to 1.5 1/d or 6 L/d because max growth rate of methanotrophs is around 0.02 1/hr, and that is the effective dilution rate of the digestor at 8 L/d. ------------------------------------------------------------------------------- Note Number: 108 (08/18/2016 14:07:57 time = 455.58887 d) Reduceing flow back to 4 L/d or 1.0 1/d dilution rate ------------------------------------------------------------------------------- Note Number: 109 (09/16/2016 13:43:23 time = 484.57181 d) Changed Air (2700 psig) and N2 (2200 psig) tanks, and calibrated instruements ------------------------------------------------------------------------------- Note Number: 110 (09/19/2016 16:53:59 time = 487.70416 d) Decrease flow to 2 L/d or 0.5 1/d algal dilution rate ------------------------------------------------------------------------------- Note Number: 111 (09/22/2016 08:19:18 time = 490.34674 d) Catostrophic failure in UV circulation loop. Almost all of the algal reactor was dumped into growth chamber. Can't fix at this moment ------------------------------------------------------------------------------- Note Number: 112 (09/22/2016 17:16:46 time = 490.71997 d) Added 4 L of new TAP medium to algal reactor and fixed plumbing problem Running at 2 L/d or 0.5 1/d as before. Also, pump now pulls from the UV loop instead of feeding into it, so that a plug will not result in UV loop over pressure ------------------------------------------------------------------------------- Note Number: 113 (10/06/2016 14:27:51 time = 504.60266 d) ------------------------------------------------------------------------------- Note Number: 114 (10/21/2016 15:49:13 time = 519.65918 d) Increased flow to 6 L/d or dilution rate of 1.5 1/d ------------------------------------------------------------------------------- Note Number: 115 (11/15/2016 14:59:37 time = 544.62476 d) Flow rate off while bioreactors were moved ------------------------------------------------------------------------------- Note Number: 116 (11/17/2016 10:43:26 time = 546.44684 d) It was noticed that the flow between reactors was cut off due to luer lok valve that had been closed flollowing reagranging reactors on 15-Nov-2016 This was fixed around 10:30 ------------------------------------------------------------------------------- Program Version: 1.21 (18-Nov-2016) Program (Re)Started: 11/18/2016 17:46:27, time = 547.74059 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting with modified version to handle additional air+co2 flow ------------------------------------------------------------------------------- Program Version: 1.21 (18-Nov-2016) Program (Re)Started: 11/18/2016 17:54:12, time = 547.74597 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restart again ------------------------------------------------------------------------------- Program Version: 1.21 (18-Nov-2016) Program (Re)Started: 11/18/2016 17:58:38, time = 547.74905 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting with out extra air+co2 flow for now ------------------------------------------------------------------------------- Note Number: 117 (11/22/2016 15:12:20 time = 551.63354 d) droping exchange flow, due to high O2 in the digestor ------------------------------------------------------------------------------- Note Number: 118 (11/22/2016 15:28:06 time = 551.64453 d) Increasing gas flow to digestor, as no flow is leaving reactor ------------------------------------------------------------------------------- Note Number: 119 (11/22/2016 15:44:56 time = 551.65619 d) trying to find leak in digestor gas ------------------------------------------------------------------------------- Note Number: 120 (11/22/2016 15:58:49 time = 551.66583 d) Changed flow rate to 20 ml/min. Seems like leak is around 15 ------------------------------------------------------------------------------- Note Number: 121 (11/23/2016 14:33:00 time = 552.60626 d) Air tank #8 online at 14:00 23-Nov-2016 ------------------------------------------------------------------------------- Program Version: 1.21 (18-Nov-2016) Program (Re)Started: 11/23/2016 14:45:03, time = 552.61462 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting with a new value for airMixAdd not set to 15.0 ------------------------------------------------------------------------------- Note Number: 122 (12/01/2016 13:42:58 time = 560.57153 d) Took UV circulation system off-line due to hardware problems. ------------------------------------------------------------------------------- Note Number: 123 (12/01/2016 13:44:36 time = 560.57263 d) Reduced N2 flow to digestor to 10 mL/min from 20 mL/min ------------------------------------------------------------------------------- Note Number: 124 (12/16/2016 16:32:39 time = 575.68933 d) Returned to Air tank #7, and used old values for O2 and CO2. At about 500 psig now ------------------------------------------------------------------------------- Program Version: 1.21 (18-Nov-2016) Program (Re)Started: 12/16/2016 16:34:17, time = 575.69047 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting after ending supplemental gas supply for SES project ------------------------------------------------------------------------------- Note Number: 125 (12/21/2016 14:06:19 time = 580.58771 d) Air tank #9 on line ------------------------------------------------------------------------------- Note Number: 126 (01/13/2017 11:43:45 time = 603.48871 d) Cell disruptor with out UV sterilizers, was placed back in line between algal reactor and digestor. No other changes where made. ------------------------------------------------------------------------------- Note Number: 127 (01/27/2017 09:53:04 time = 617.41187 d) Flow between reactors was blocked due to flow from algae to digestor clog. Freed and now flowing again. ------------------------------------------------------------------------------- Note Number: 128 (03/09/2017 19:01:49 time = 658.79291 d) Recalibrated older air tank#7. Note, CO2 reading may be low due to my error. Should have been closer to 2.09 based on old reading ------------------------------------------------------------------------------- Note Number: 129 (03/15/2017 17:51:45 time = 664.74426 d) Air tank #10 on-line at 17:20 15 Mar 2017. 2500 psig ------------------------------------------------------------------------------- Note Number: 130 (03/15/2017 17:52:34 time = 664.74481 d) back to sampling after air tank 10 change and calibration ------------------------------------------------------------------------------- Note Number: 131 (03/23/2017 10:09:29 time = 672.42328 d) Increase flow rate to 8 L/d, or a dilutioin rate of 2 1/d. This might cause washout of the methanotrophs, but no methanogenesis is currently occuring! ------------------------------------------------------------------------------- Note Number: 132 (03/23/2017 10:10:54 time = 672.42426 d) Sampling after increasing dilution rate to 2 1/d ------------------------------------------------------------------------------- Note Number: 133 (03/29/2017 10:35:39 time = 678.44141 d) N2 tank went empty some time yesterday or this morning. Just replaced at 10:35 29 Mar 2017. ------------------------------------------------------------------------------- Note Number: 134 (04/06/2017 09:58:12 time = 686.41541 d) New N2 tank online 2250 psig ------------------------------------------------------------------------------- Note Number: 135 (04/18/2017 09:53:04 time = 698.41187 d) Methane analyzer has malfunctioned. Still trying to diagnose ------------------------------------------------------------------------------- Program Version: 1.21 (18-Nov-2016) Program (Re)Started: 08/16/2017 16:31:24, time = 818.68847 d Sample loop purge time for CH4mix (min): 12.00; On port: 10 Sample loop purge time for Air (min): 6.00; On port: 10 Sample loop purge time for Algae (min): 6.00; On port: 1 Sample loop purge time for N2 (min): 6.00; On port: 10 Sample loop purge time for Dig (min): 6.00; On port: 2 Loop time (hr): 2.00 --------------------------- Experiment Description ---------------------------- Restarting after very long period while CH4 analyzer was repaired -------------------------------------------------------------------------------